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Genotypic characterization of psittacid herpesvirus isolates from Brazil

机译:来自巴西的伪酸疱疹病毒分离株的基因型鉴定

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Thirty-six isolates of psittacid herpesvirus (PsHV), obtained from 12 different species of psittacids in Brazil, were genotypically characterized by restriction fragment length polymorphism (RFLP) analysis and PCR amplification. RFLP analysis with the PstI enzyme revealed four distinct restriction patterns (A1, X, W and Y), of which only A1 (corresponding to PsHV-1) had previously been described. To study PCR amplification patterns, six pairs of primers were used. Using this method, six variants were identified, of which, variants 10, 8, and 9 (in this order) were most prevalent, followed by variants 1, 4, and 5. It was not possible to correlate the PCR and RFLP patterns. Twenty-nine of the 36 isolates were shown to contain a 419 bp fragment of the UL16 gene, displaying high similarity to the PsHV-1 sequences available in GenBank. Comparison of the results with the literature data suggests that the 36 Brazilian isolates from this study belong to genotype 1 and serotype 1.
机译:通过限制性片段长度多态性(RFLP)分析和PCR扩增从基因型上鉴定了从巴西的12种不同的齿酸中分离出的36种齿酸疱疹病毒(PsHV)。用PstI酶进行的RFLP分析揭示了四个不同的限制性酶切模式(A1,X,W和Y),其中先前仅描述了A1(对应于PsHV-1)。为了研究PCR扩增模式,使用了六对引物。使用此方法,鉴定出六个变体,其中变体10、8和9(按此顺序)最普遍,其次是变体1、4和5。不可能将PCR和RFLP模式关联起来。已显示36个分离物中的29个包含UL16基因的419 bp片段,与GenBank中可用的PsHV-1序列具有高度相似性。结果与文献数据的比较表明,来自该研究的36个巴西分离株属于基因型1和血清型1。

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