Reversed phase ultra performance liquid chromatography method for determination of bimatoprost from active pharmaceutical dosage form

摘要

A reversed phase ultra performance liquid chromatography method was developed and validated for the assay of bimatoprost from active pharmaceutical ingredients. The separation of drug was achieved on Acquity BEH C18 (50mm X 2.1 mm) 1.7- column. The mobile phase was buffer and acetonitrile in the ratio of 60:40 % v/v. The buffer was used 0.002 M pentane sulphonic acid sodium salt monohydrate .UV detection was performed at 210 nm. The validation of method was done as per ICH guideline using parameters like, specificity, linearity, accuracy, precisions, robustness, solution stability of sample. The developed method was used for analysis of bimatoprost from bulk drug and pharmaceutical dosage form.