A Comparison of Chemical Composition, Antioxidant and Antimicrobial Studies of Abutilon indicum Leaves and Seeds

摘要

Objective: The objective of this study was to evaluate in vitro antioxidant, antimicrobial activity and characterize the chemical constituents of leaves and seeds of Abutilon indicum by using gas chromatography-mass spectrometry (GC-MS). In addition total phenolics and flavanoids were also estimated. Materials and Methods: The GC-MS analysis of plant and seed extracts was performed using a GC-Brucker 436 system and interpretation on mass spectrum GC-MS was conducted using the database of National Institute Standard and Technology (NIST). Antioxidant activity was determined in the 1,1-diphenyl-2-picrylhydrazyl (DPPH) method. The total flavonoids assay was conducted by aluminium chloride colorimetric method and otal phenolics content was determined according to the Folin-Ciocalteu method. Antibacterial activity and minimum inhibitory concentration s were calculated by the agar diffusion method. Results: Twenty five chemical compounds are identified from the plant extract and 29 compounds identified from seed extract by GC-MS analysis. The leaf and seed extracts showed significant DPPH scavenging activity (IC₅₀ values are 48.94±3.54 and 49.89±3.29 μg mL^?1) compared with the values obtained for ascorbic acid standard (IC₅₀ value 39.22±5.50). The leaf and seed extracts prevented the growth of both Gram-positive and Gram-negative bacteria. The antimicrobial and antioxidant activities of the extracts were positively associated with the total phenolic and flavonoid contents of the extract. Conclusion:The presence of various bioactive compounds justifies the use of Abutilon indicum for various ailments by traditional practitioners.