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Poly (ethylene glycol) hydrogel elasticity influences human mesenchymal stem cell behavior

机译:聚乙二醇水凝胶弹性影响人间充质干细胞的行为

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Coordinated investigations into the interactions between biologically mimicking (biomimetic) material constructs and stem cells advance the potential for the regeneration and possible direct replacement of diseased cells and tissues. Any clinically relevant therapies will require the development and optimization of methods that mass produce fully functional cells and tissues. Despite advances in the design and synthesis of biomaterial scaffolds, one of the biggest obstacles facing tissue engineering is understanding how specific extracellular cues produced by biomaterial scaffolds influence the proliferation and differentiation of various cell sources. Matrix elasticity is one such tailorable property of synthetic scaffolds that is known to differ between tissues. Here, we investigate the interactions between an elastically tailorable polyethylene glycol (PEG)-based hydrogel platform and human bone marrow-derived mesenchymal stem cells (hMSCs). For these studies, two different hydrogel compositions with elastic moduli in the ranges of 50–60?kPa and 8–10?kPa were implemented. Our findings demonstrate that the different elasticities in this platform can produce changes in hMSC morphology and proliferation, indicating that the platform can be implemented to produce changes in hMSC behavior and cell state for a broad range of tissue engineering and regenerative applications. Furthermore, we show that the platform’s different elasticities influence stem cell differentiation potential, particularly when promoting stem cell differentiation toward cell types from tissues with stiffer elasticity. These findings add to the evolving and expanding library of information on stem cell–biomaterial interactions and opens the door for continued exploration into PEG-based hydrogel scaffolds for tissue engineering and regenerative medicine applications.
机译:对生物模仿(仿生)材料结构与干细胞之间相互作用的协调研究,为患病细胞和组织的再生和可能的直接替代提供了可能。任何与临床相关的疗法都需要开发和优化可大量生产功能齐全的细胞和组织的方法。尽管在生物材料支架的设计和合成方面取得了进展,但组织工程面临的最大障碍之一是了解生物材料支架产生的特定细胞外信号如何影响各种细胞来源的增殖和分化。基质弹性是已知的在组织之间不同的合成支架的这种可定制的特性之一。在这里,我们研究了基于弹性可调节的聚乙二醇(PEG)的水凝胶平台与人类骨髓间充质干细胞(hMSCs)之间的相互作用。对于这些研究,使用了两种不同的弹性模量在50–60?kPa和8–10?kPa范围内的水凝胶。我们的发现表明,该平台中的不同弹性可能会导致hMSC形态和增殖发生变化,这表明该平台可实现为hMSC行为和细胞状态产生变化,适用于广泛的组织工程和再生应用。此外,我们证明了平台的不同弹性会影响干细胞分化的潜力,特别是当从具有更强弹性的组织促进干细胞向细胞类型的分化时。这些发现增加了有关干细胞-生物材料相互作用的信息库,从而不断发展,为继续探索用于组织工程和再生医学应用的基于PEG的水凝胶支架打开了大门。

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