Effect of water spinach and biochar on methane production in an in vitro system with substrate of Bauhinia acuminata or Bitter Neem (Azadirachta indica) leaves

摘要

Theobjectiveofthisstudywastodeterminetheeffectofwaterspinachassourceofsolubleprotein,andofbiocharassupportforbiofilms,onmethaneproductioninaninvitroincubationsystemusingleavesofBauhiniaacuminataandAzadirachtaindicaasthesubstrate.Foreachsourceofleaves,theexperimentaldesignwasa2*2factorialarrangementof4treatmentswithfourreplications.Measurementsweremadeoftotalgas,percentmethaneinthegasandDMandNsolubilized,atintervalsof6,12,18and24h.GasproductionandpercentsubstratesolubilizedwereincreasedbywaterspinachonbothBauhiniaandBitterNeem.Bycontrast,waterspinachdidnotaffectmethaneproductiononBauhiniabutincreaseditonBitterNeem.Biocharsupplementationhadnoeffectontotalgasandmethaneproduction.