Methane production from urea-treated rice straw is reduced when the protein supplement is cassava leaf meal or fish meal compared with water spinach meal in a rumen in vitro fermentation

摘要

Theaimofthisstudywastoevaluatetheeffectofprotein-richfeedsresistanttofermentationintherumeninreducingmethaneproductioninaninvitroincubationsystem.Thetreatmentsinacompletelyrandomizedblockdesign(RCBD)with4treatmentsand4replicationsofeachtreatmentwereUTR:Urea-treatedricestrawwithadditionalureatoprovideapproximately15%crudeproteininthesubstrateDM;WS:sameasUTRwithwaterspinachmealatlevelof6gcrudeproteinper100gsubstrateDM;FM:sameasUTRwithfishmealatlevelof6gcrudeproteinper100gsubstrateDM;CLM:sameasUTRwithcassavaleafmealatlevelof6gcrudeproteinper100gsubstrateDM.ThequantityofsubstrateDMineachfermentationbottlewas12gtowhichwereadded240mlrumenfluid(fromslaughteredbuffalo)and960mlofbuffersolution.Theincubationwasfor24hwithmeasurementsoftotalgasproductionandmethanepercentageatintervalsof6,12and24hoursanddeterminationofresidualDMunfermentedsubstrateattheend.Onallthetreatments,theconcentrationofmethaneinthegasincreasedexponentiallywiththedurationoftheincubation,highestvaluesbeingrecordedforthewaterspinachtreatmentandlowestvaluesforthecassavaleafmeal.Methaneproductionwasreducedwhenthecrudeproteinsourcewasfishmealorcassavaleafmealascomparedwithwaterspinach.Theimplicationsarethatmethaneproductionisreducedwhenthedietarycrudeproteincomponentislessrumendegradable.