Genetic characterisation in snapper (Lutjanus goreensis) and grunter (Pomadasys jubelini) populations from high and low brackish lagoons using randomly amplified polymorphic DNA technique

摘要

Snapper(Lutjanusgoreensis)andGrunter(Pomadasysjubelini)populationsfromahigh(LagosLagoon)andlowbrackishlagoon(BadagryLagoon)werestudiedtoassesstheirgeneticrelationshipusingrandomlyamplifiedpolymorphicDNAanalysis(RAPD)technique.Thisinvolvedtheuseof10-merOPASprimerstoassaypolymorphismsbetweenthetwopopulationsofbothspecies.VaryingRAPDfragmentpatternswereobservedfordifferentlocations.Ofthetwoprimersutilized,onlyoneproduceddistinctandconsistentRAPDprofilesforbothspeciesfromthetwolocations.Forty-nine(49)andthirty-six(36)reproduciblebandsrepresentingslight(25.8%and20.0%)DNApolymorphismsinL.goreensisandP.jubeliniwereuncoveredinpopulationsfrombothlocations.ClusteranalysisperformedtocreatedendrogramsusingUPGMAbythePhyllipsoftwaregavenosufficientgeneticdivergencetodiscriminatethesamplesofdifferentpopulations.Theresultofthisresearchisveryimportantbecauseitpresentsbaselineinformationonthegeneticdiversityofsnappersandgruntersfromthesetwowaterbodies.