Pre‐ mRNA splicing is modulated by antifungal drugs in the filamentous fungus Neurospora crassa

摘要

For this study, we sought to identify pre‐ mRNA processing events modulated by changes in extracellular pH , inorganic phosphate, and antifungal drugs. We examined genes with at least four putative introns whose transcriptional level responded to these effectors. We showed that the intron retention levels of genes encoding asparagine synthetase 2, C6‐zinc finger regulator ( fluffy ), and a farnesyltransferase respond to amphotericin B, ketoconazole, and other effectors. In general, the assayed antifungals promoted the disruption of the structural domains of these proteins probably leading to their inactivation, which emphasize the complexity of the metabolic modulation exerted by antifungal signaling. Here, Mendes et al. show that intron retention by the genes coding for asparagine synthetase 2 ( asn‐2 ), C6‐zinc finger regulator ( fluffy ), and a farnesyltransferase ( ram‐1 ) in the filamentous fungus Neurospora crassa occurs in response to extracellular pH and Pi changes and the presence of antifungal drugs. These results emphasize the complexity of the metabolic modulation exerted by antifungal signalling.