Seed set and some cytological characters in different generations of autotetraploid perennial rye (Secale montanum Guss)

摘要

Abstract Artificial autotetraploids of diploid perennial rye (Secale montanum Guss) plants were obtained by colchicine treatment. C1 C2 and C3 generations of autotetraploid perennial rye were compared for seed set and cytological characteristics such as irregularities in metaphase I (univalents) and anaphase I and II (lagging chromosomes and/or chromatids and bridge formation) and micronuclei in tetrads. In the three generations (C1 C2 and C3) the percentage of cells with univalents was 18.51, 14.56 and 9.38% respectively. The percentage of cells with irregular anaphase I with univalents was 23.43, 15.93 and 7.85%, and for anaphase II cells it was 20.93, 16.47 and 7.16. The percentage of tetrads with micronuclei was 18.17, 14.07 and 6.17% and seed set was 27.72, 43.51 and 59.59% respectively. A negative correlation was found between seed set and the cytological characteristics investigated. The correlations among the characters investigated during meiosis division were statistically significant. Selection...