First report of Tomato leaf curl virus and Croton yellow vein mosaic betasatellite infecting chilli plants in Pakistan

摘要

Leaf curl disease poses a major constraint to chilli (Capsicum annuum) production in Pakistan. In October 2014, chilli plants exhibiting geminivirus-like symptoms, including leaf curling and stunting were observed at the National Agriculture Research Centre (Islamabad, Pakistan), with an incidence of 50%. Leaf samples were collected from ten diseased chilli plants. The samples were tested by TAS-ELISA using a panel of ten monoclonal antibodies (Harrison et al., 1997). A total of eight monoclonal antibodies indicated the presence of begomoviruses in five field samples.