Bacteriological Profile of Sepsis and Their Antibiotic Susceptibility Pattern in Adult Patients in a Tertiary Care Hospital of Madhya Pradesh, India

摘要

"Introduction: Blood stream infections can lead to sepsis. Severe sepsis is one of the leading causes of death. Both gram positive and negative bacteria are important etiological agents for sepsis. Bacteria causing sepsis show multi-drug resistance which increases the morbidity and mortality in sepsis patients. Objectives: To isolate the bacteria causing sepsis and their antibiotic susceptibility pattern in adult patients. Material and methods: A total number of 296 blood samples of adult patients with sepsis were taken and processed as per standard protocol. Identification of bacteria was carried out according to the standard biochemical tests. Antimicrobial susceptibility test was carried out on Mueller Hinton agar plates by the Kirby- Bauer disk diffusion method, according to the Central Laboratory Standards Institute guidelines. Results: Bacteria were isolated in 79 samples (26.69%). 54 isolates (68.35%) were gram negative bacilli and 25 isolates (31.65%) were gram positive cocci. Klebsiella spp. and Staphylococcus aureus were the predominant isolates among gram negative and gram positive bacteria respectively. Imipenem and linezolid were the most sensitive antibiotics for gram negative and gram positive bacteria respectively while ampicillin showed maximum resistance. Conclusion: Gram negative bacilli were more common for causing sepsis in adults. As the culture positivity is quite less by conventional method, other methods for isolation of bacteria should be considered. Multi-drug resistance is shown by most of the causative bacteria and may be an important factor for high mortality in sepsis patients. Selection of antibiotics for the treatment of sepsis patients should be individualized to improve outcome.