The Arabidopsis Downy Mildew Resistance Gene, RPP13-Nd, Functions Independently of NDR1 and EDS1 and Does Not Require the Accumulation of Salicylic Acid

摘要

RPP13-Nd -mediated resistance prevents parasitism by five isolates of Peronospora parasitica ( At ) in a transgenic Arabidopsis. Columbia background. We tested the effect of a number of known disease resistance mutations on the RPP13-Nd function and found that resistance remained unaltered in plants carrying mutations in either EDS1 or NDR1 and in double ndr1-1/eds1-2 mutant lines. Furthermore, we found that pbs2, pad4-1, npr1-1 , and rps5-1 , which compromise resistance to a number of P. parasitica (At) isolates, had no affect on RPP13-Nd function. In addition, RPP13-Nd -mediated resistance remained unchanged in a background of salicylic acid depletion ( nahG ). We conclude that RPP13-Nd is the first Arabidopsis R gene product reported to act via a novel signaling pathway that is independent of salicylic acid-mediated responses and is completely independent of NDR1 and EDS1.