Combining chondroitinase ABC and growth factors promotes the integration of murine retinal progenitor cells transplanted into Rho?/? mice

摘要

Purpose: The aim of this study is toinvestigate the synergistic effect of chondroitinase ABC and growthfactors in the integration of murine retinal progenitor cells (mRPCs)transplanted into Rho?/? mice. Methods: mRPCs from P1 green fluorescentprotein-transgenic mice were isolated and expanded for transplantation.All mRPCs of 20 passages or less were transplanted into the subretinalspace of B6 mice together with chondroitinase ABC, and into Rho?/?mice combined with chondroitinase ABC, N-[N-(3,5-Difluorophenacetyl)-L-alanyl]-S-phenylglycine t-butyl ester (DAPT),and insulin growth factor (IGF)-1. Cell counts were used to examine themigration and survival rate of mRPCs in B6 mice. Immunohistochemistrywas used to evaluate the differentiation and integration of mRPCs in B6and Rho?/? mice. Results: Our results show thatsubstantial numbers of mRPCs migrated and survived in the retina whentransplanted with chondroitinase ABC into B6 and Rho?/?mice. Chondroitinase ABC disrupted the glial scar around the mRPCs inthe subretinal space. Only a few mRPCs expressed recoverin in B6 mice.More mRPCs expressed rhodopsin, recoverin, and synaptophysin aftertransplantation into Rho?/? mice when combined withchondroitinase ABC and growth factors. Conclusions: The synergistic effect ofchondroitinase ABC and growth factors facilitates the anatomicintegration of mRPCs transplanted into Rho?/? mice.