Gene Editing and Genome Engineering with CRISPR-Cas9This article was transcribed from a presentation delivered by Professor Charpentier at the Molecular Frontiers Symposium on Tailored Biology at the Royal Swedish Academy of Sciences, May 2017. It is published here in MFJ with permission from Professor Charpentier.

摘要

The RNA-programmable CRISPR-Cas9 technology allows precise and efficient engineering or correction of mutations, modulation of gene expression and marking of DNA in a wide variety of cell types and organisms in the three domains of life. Because of its versatility and ease of design, this powerful technology has been rapidly and universally adopted for genome editing applications in life science research. It is also recognized for its promising and potentially transformative applications in biotechnology, medicine and agriculture.