Whole genome sequence of Klebsiella pneumoniae U25, a hypermucoviscous, multidrug resistant, biofilm producing isolate from India

摘要

Klebsiella pneumoniae U25 is a multidrug resistant strain isolated from a tertiary care hospital in Chennai, India.Here, we report the complete annotated genome sequence of strain U25 obtained using PacBio RSII. This is thefirst report of the whole genome of K. pneumoniae species from Chennai. It consists of a single circular chromosomeof size 5,491,870-bp and two plasmids of size 211,813 and 172,619-bp. The genes associated with multidrug resistancewere identified. The chromosome of U25 was found to have eight antibiotic resistant genes [blaOXA-1, blaSHV-28,aac(6’)1b-cr, catB3, oqxAB, dfrA1]. The plasmid pMGRU25-001 was found to have only one resistant gene (catA1)while plasmid pMGRU25-002 had 20 resistant genes [strAB, aadA1, aac(6’)-Ib, aac(3)-IId, sul1,2, blaTEM-1A,1B,blaOXA-9, blaCTX-M-15, blaSHV-11, cmlA1, erm(B), mph(A)]. A mutation in the porin OmpK36 was identifiedwhich is likely to be associated with the intermediate resistance to carbapenems in the absence of carbapenemasegenes. U25 is one of the few K. pneumoniae strains to harbour clustered regularly interspaced short palindromic repeats(CRISPR) systems. Two CRISPR arrays corresponding to Cas3 family helicase were identified in the genome.When compared to K. pneumoniae NTUHK2044, a transposase gene InsH of IS5-13 was found inserted.