STUDY ON THE BIODEGRADATION CHARACTERISTICS OF ISOFENPHOS-METHYL AND ISOLATION OF AN ISOFENPHOS-METHYL DEGRADING BACTERIUM, BACILLUS ATROPHAEUS IM-5

摘要

A strain IM-5 capable of highly degrading isofenphos-methyl was isolated from soil. The strain IM-5 was identified as Bacillus atrophaeus based on 16S rRNA and analysis of morphology, physiological and biochemical characters. The optimal pH value and temperature of strain IM-5 were 7.0 and 35-40°C. The strain IM-5 also had high tolerance to isofenphos-methy. The degradation rate of isofenphos-methy (50mg/L) by the strain IM-5 could reach about 93.2% in 5d. Isopropyl salicylate and salicylic acid were two important intermediate metabolites of isofenphos-methy biodegradation. The strain IM-5 could degrade isopropyl salicylate quickly, but could not degrade salicylic acid.