DETECTION OF BCMV, AMV AND CMV BY DAS-ELISA AND IMMUNOCAPTURE-RT-PCR IN BEAN-GROWING AREAS IN THE WEST MEDITERRANEAN REGION, TURKEY

摘要

This study was conducted to detect viral diseases in bean growing areas in the West Mediterranean region of Turkey. In the surveys during two years, a total of 298 (100 samples in 2011 and 198 samples in 2012) plants with symptoms were collected from 17 localities. These samples were tested by double antibody sandwich enzyme linked immunosorbent assay (DAS-ELISA) for Bean common mosaic virus (BCMV), Cucumber mosaic virus (CMV) and Alfalfa mosaic virus (AMV). Infection percentages of the viruses were 35.91%, 9.06% and 5.70% for CMV, BCMV and AMV, respectively. Among detected mixed infections, BCMV+CMV was the most common status with an incidence of 16.44%. Twenty five leaf samples, which gave positive reaction in DAS-ELISA, were used for immunocapture-reverse-transcription-polymerase chain reaction (IC-RT-PCR) which was resulted in amplification fragments in expected size of 850, 678 and 351 bp for BCMV, CMV and AMV, respectively. The presence of BCMV, CMV and AMV on bean plants in the West Mediterranean region of Turkey was confirmed by DASELISA and IC-RT-PCR using specific primers.