Genetic distance based on SSR and grain yield of inter and intrapopulational maize single cross hybrids.

摘要

The objective of this work was to correlate the genetic distances between the progenitors obtained by microsatellite markers with the grain yield of inter and intrapopulational maize single cross hybrids. Three S0 populations derived from commercial single cross hybrids were used to obtain 163 hybrids (110 interpopulational and 53 intrapopulational). The two best hybrids and two worst hybrids of each the inter- and intrapopulational crosses were selected and their progenitors maintained through self-pollination of the second ear of each S0 plant, genotyped with 47 SSRs. The Modified Roger’s Distance (MRD) between each pair of S1 inbred lines, the number of alleles and the polymorphic information content (PIC) of each primer were estimated. The genetic distances between progenitors were correlated with the grain yield of the inter- and intrapopulational hybrids. The number of obtained alleles was 186, with a mean of 3.96 alleles. The PIC varied from 0.49 to 0.80, with a mean of 0.65. The mean genetic distance between all S1 inbred lines was 0.75, varying from 0.40 to 0.89, indicating the existence of variability between the S1 inbred lines. The correlation between MRD and grain yield was high and significant for the interpopulational crosses (r = 0.84, P ≤ 0.01) and low and not significant (r = 0.18, P ≥ 0.05) for intrapopulational crosses.