Validation of quantitative polymerase chain reaction with Southern blot method for telomere length analysis

摘要

Aim: Telomere length (TL) measurement by quantitative polymerase chain reaction (PCR) has been widely accepted, but limited information regarding its validation with a gold-standard technique is available. Materials & methods: We measured TL by Southern blot and monochrome multiplex quantitative PCR (MMqPCR) and validated the results of TL in leukocytes of 94 participants with mean age 43.2?years, BMI 19–41 (mean 27.8?±?4.3) kg/m2. Results: A significant positive correlation was observed between TL measured by MMqPCR and Southern blot assay (correlation coefficient r?=?+0.896, p?