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首页> 外文期刊>Frontiers in Microbiology >Identification and Characterization of Mycoplasma feriruminatoris sp. nov. Strains Isolated from Alpine Ibex: A 4th Species in the Mycoplasma mycoides Cluster Hosted by Non-domesticated Ruminants?
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Identification and Characterization of Mycoplasma feriruminatoris sp. nov. Strains Isolated from Alpine Ibex: A 4th Species in the Mycoplasma mycoides Cluster Hosted by Non-domesticated Ruminants?

机译: feriruminatoris sp的鉴定和表征。十一月从高山高地山羊中分离的菌株:由非驯养的反刍动物寄养的 Mycoplasma mycoides 簇中的第四种?

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摘要

The genus Mycoplasma , a group of free-living, wall-less prokaryotes includes more than 100 species of which dozens are primary pathogens of humans and domesticated animals. Mycoplasma species isolated from wildlife are rarely investigated but could provide a fuller picture of the evolutionary history and diversity of this genus. In 2013 several isolates from wild Caprinae were tentatively assigned to a new species, Mycoplasma ( M.) feriruminatoris sp. nov., characterized by an unusually rapid growth in vitro and close genetic proximity to ruminant pathogenic species. We suspected that atypical isolates recently collected from Alpine ibex in France belonged to this new species. The present study was undertaken to verify this hypothesis and to further characterize the French ibex isolates. Phylogenetic analyses were performed to identify the isolates and position them in trees containing several other mycoplasma species pathogenic to domesticated ruminants. Population diversity was characterized by genomic macrorestriction and by examining the capacity of different strains to produce capsular polysaccharides, a feature now known to vary amongst mycoplasma species pathogenic to ruminants. This is the first report of M. feriruminatoris isolation from Alpine ibex in France. Phylogenetic analyses further suggested that M. feriruminatoris might constitute a 4th species in a genetic cluster that so far contains only important ruminant pathogens, the so-called Mycoplasma mycoides cluster. A PCR assay for specific identification is proposed. These French isolates were not clonal, despite being collected in a restricted region of the Alps, which signifies a considerable diversity of the new species. Strains were able to concomitantly produce two types of capsular polysaccharides, β-(1→6)-galactan and β-(1→6)-glucan, with variation in their respective ratio, a feature never before described in mycoplasmas.
机译:支原体属是一组自由生活的无壁原核生物,包括100多种,其中数十种是人类和家养动物的主要病原体。从野生生物中分离出的支原体种类很少进行调查,但可以提供该属的进化历史和多样性的更全面的描述。在2013年,一些来自野生Caprinae的分离株被暂定分配给一个新物种Feriruminatoris sp。的支原体。 nov。,其特征是在体外异常快速生长,并且与反刍动物致病物种的遗传距离非常近。我们怀疑最近从法国的高山高地山羊中收集到的非典型分离株属于这一新物种。进行本研究以验证该假说并进一步表征法国ibex分离株。进行了系统进化分析,以鉴定分离株并将其定位在含有对驯化反刍动物致病的其他几种支原体物种的树中。群体多样性的特征在于基因组的宏观限制和研究不同菌株产生荚膜多糖的能力,而荚膜多糖是目前已知的对反刍动物致病的支原体物种之间差异的特征。这是法国首次从高山高地山羊中分离出feriruminatoris的报道。系统发育分析进一步表明,feriruminatoris可能构成一个遗传簇中的第四个物种,该簇迄今仅包含重要的反刍动物病原体,即所谓的Mycoplasma mycoides簇。提出了用于特异性鉴定的PCR测定法。尽管这些法国分离株是在阿尔卑斯山的一个限定区域收集的,但它们并不是克隆的,这表明新物种的多样性。菌株能够伴随地产生两种类型的荚膜多糖,即β-(1→6)-半乳聚糖和β-(1→6)-葡聚糖,它们各自的比例有所变化,这是支原体中从未有过的特征。

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