Early Pregnancy Diagnosis in Water Buffalo by Early Pregnancy Factor Measurement Using Rosette Inhibition Test

摘要

The diagnosis of pregnancy is essential for profitable animal husbandry especially in the productiveanimal species. Early pregnancy factor (EPF) was detected in the serum of all mammals tested within 24 to 48h of fertilization and disappeared within 24 to 48 h after death or removal of embryo. EPF activity may be usefulas an indirect method of pregnancy diagnosis. Blood samples from 50 artificially inseminated (AI) and 10 noninseminated(control) Iranian water buffalo (Bubalusbubalis) were collected and allowed to clot. Serum wascollected and stored at -20°C for assay. Test of pregnancy (rectal palpation) was done after 50th day. Rosetteinhibition test (RIT) was performed to evaluate the EPF activity. RIT values were significantly higher (P < 0.05)in pregnant (8.00 ± 0.13) than in non-pregnant (2.71 ± 0.21) cows. In conclusion, the RIT score for EPF detectioncan be used as a tool for early detection of pregnancy or embryonic death in buffalos.