Effects of Oxidative Stress Induced by Antouka Super? (Insecticide) on Some Reproductive Parameters of Male Japanese Quail ( Coturnixcoturnix japonica ) and Mitigation Strategies Using Aqueous Leaves Extract of Persea americana

摘要

In this study, the effect of oxidative stress induced by Antouka Super? on some reproductiveparameters of male Japanese quail (Coturnixcoturnix japonica) and mitigation strategies using aqueous leavesextract of Persea americana were studied.Forty immature male Japanese quails (28 days old), were divided intofive groups of 8 animals each subjected to the following treatments: Group 1, birds receivingdistilledwater(negative control group: CO-); Group 2, birds receiving 75 mg of Antouka Super?/kg b.w(positive controlgroup: CO+); while groups 3, 4 and 5 were administered 50, 100 and 200 mg/ kg b.w of aqueous leaves extractof Persea americana (AEPA) respectively together with antouka super at 75 mg/kg. All the test solutions wereorally administered once a day for 60 days usingendogastriccanule. Dissection of the vas deferens wasperformed to obtain sperm cells. The protective effects of AEPA on the organ weight, oxidative stressbiomarkers, serum hormones and sperm characteristics were evaluated. The results revealed that exposure toAntouka Super? significantly (p<0.05) decreased organ weight (testis, epididymis and vas deferens), levels oftesticular proteins and serum hormones (LH, FSH and Testosterone). This insecticide also significantly (p<0.05)decreased sperms mobility, viability and density and increasedsperm abnormalities (minor and major). Inaddition, the activities of superoxide dismutase (SOD), total peroxidase (POD) and catalase (CAT) significantlydecreased (p<0.05) in the testis; while malondialdehyde (MDA) significantly increased (p<0.05) compared tothe values recorded in birds of negative control group. Administration of AEPA to treated-animals alleviatesthe reproductive toxicity and testicular oxidative damage induced by Antouka Super?. Inconclusion, exposureofmale Japanese quail to Antouka Super? induce oxidative stress and deteriorate reproductive parameters;effects that can be alleviated using aqueousleaves extract of Persea americana.