Effect of Different Concentrations of DMSO and Dilution Rates on Viability of Beluga ( Huso huso ) Post-Thawed Sperm

摘要

Milt obtained from four Beluga males (Huso huso) was cryopreserved using extender; Tris-sucrose-KCl (30mM Tris, 23.4mM sucrose, 0.25mM KCl, PH 8.0) supplemented with DMSO at concentration of 5%, 10%and 20%. Semen was diluted, respectively, with ratios of 1:0.5, 1:1 and 1:2 with extender and frozen in liquidnitrogen vapor. Frozen sperms after 30 and 60 days were excluded from freezing. Experiment showed the highestmotility duration and the most motility percentage of post-thawed sperms after 30 days was related to thetreatments with the concentration ofDMSO 10% and the dilution ratio of 1: 1 (129±24.52s and12.63±4.52%;P<0.05), as well as the upmost mobility and the motility duration of post-thawed sperms after 60 days wasrelated to the treatments with the concentration of DMSO 10% and the dilution ratio of 1: 1 (90.33±22.52 sand10.08±4.32%; P<0.05). The results showed that holding time from 30 to 60 days of frozen sperm has anegative impact on Motility duration and Motility percentage of post-thawed sperms. Results also showed thathighest motility duration and motility percentage of post-thawed sperms was for treatments that have beendiluted with 1:1 ratio.