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RNA sequencing analysis of gene expression regulated by the transcription factor SlZFP2 during early fruit development

机译:果实发育早期受转录因子SlZFP2调控的基因表达的RNA测序分析

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The transcription factor SlZFP2 ( Solanum lycopersicum Zinc Finger Protein 2 ) regulates ABA biosynthesis during fruit development. To reveal the regulatory network of this transcription factor, we conducted a high-throughput RNA-seq to identify differentially expressed genes in 2 dpa (days post anthesis) fruits from a representative RNAi line in Solanum pimpinellifolium LA1589 background and the wild type. The transcriptome analysis revealed that expression of 2722 genes was regulated by SlZFP2 during early fruit development and further helped to narrow down its direct targets to 193 genes. Here, we provide a detailed description of the experimental procedure associated with our transcriptome sequencing data deposited in the National Center for Biotechnology Information (accession no. GSE63838).
机译:转录因子SlZFP2(番茄茄属锌指蛋白2)在果实发育过程中调节ABA的生物合成。为了揭示该转录因子的调控网络,我们进行了高通量RNA-seq鉴定,从2 dpa(花后几天)的果实中提取了Pimpinellifolium茄LA1589背景和野生型中代表RNAi系的差异表达基因。转录组分析显示,在果实发育早期,SlZFP2调节了2722个基因的表达,并进一步帮助将其直接靶标范围缩小到193个基因。在这里,我们将详细介绍与保存在国家生物技术信息中心(登录号GSE63838)中的转录组测序数据相关的实验程序。

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