Analyses of genetic diversity of Badis badis (Hamilton-Buchanan 1822) from three Riverine systems in sub-Himalayan biodiversity hotspot of west Bengal, India using RAPD and ISSR fingerprinting

摘要

Badis badis is a threatened freshwater fish in the Indian scenario, and the population genetic architecture of this fish is largely unexplored in the eastern sub-Himalayan biodiversity hotspot of West Bengal, India, also known as the Terai and the Dooars. Total seventeen populations from three major river systems viz. Mahananda (Terai), Teesta and Jaldhaka (Dooars) have been studied through RAPD and ISSR fingerprinting. The polymorphism, genetic diversity and Shannon’s Information index were calculated for each population. The highest values for Nei’s genetic diversity (0.1436± 0.1963 and 0.1409 ±0.1954 after RAPD and ISSR analyses) and Shannon’s information index (0.2150±0.2794 and 0.2109 ±0.2785 after RAPD and ISSR analyses) were found in Jaldhaka river system. The UPGMA dendrogram revealed that the Mahananda and Teesta populations form a single group and the Jaldhaka population forms a separate group. Comparing with other related studies the genetic diversity Badis badis was found to be low in the three main riverine systems of the Terai and Dooars region of West Bengal, although the Jaldhaka population showed a comparatively higher level of genetic diversity. Therefore, Jaldhaka population should be managed and, conserved to preserve the available gene pool of this threatened species in this region. Low levels of genetic diversity were found in the present study among the seventeen populations validating the recent assignment of its threatened status.