Identification of QTLs associated with low chloride accumulation in oriental tobacco

摘要

The construction of linkage maps and identification of genomic regions controlling traits have great significance for plant breeders. Among different chemical traits in oriental tobacco, chloride content in leaves when it is less than 1.5% improves burning quality. In this study, genetic analysis of quantitative trait loci affecting the chloride accumulation of oriental tobacco leaf was performed using an F2 population of a cross between two oriental tobacco genotypes, ‘Basma Seres 31’ and ‘SPT 406’, comprising 100 individuals. A normal distribution was observed for the chloride accumulation in the F2 population. Linkage map with 23 informative microsatellite and 29 inter simple sequence repeat markers was constructed, which covered 570.8 cM of the tobacco genome. Single marker analysis, interval mapping and composite interval mapping were used to detect the putative QTLs controlling chloride accumulation. There was not any significant relation between ISSR markers and chloride accumulation. SSR marker PT30346 was found to be significantly associated with chloride accumulation through single marker analysis. Two QTLs including ChlIM and ChlCIM with R2 values of 0.4 and 0.07 were identified using IM and CIM, respectively.