Tatiana Yudina, Elena Sorokina, Vadim Danilov

摘要

We have investigated the effect of streptomycin simultaneously on the luminescence and the growth of bacteria of luminescent recombinant Escherichia coli stain with the operon luxCDABE from Vibrio fischeri. Experiments were performed to determine the toxicity in the 30-minute experiment and in the chronic 24-hour variant. The level of toxicity in the tested concentration range was determined judging by the change of intensity of bioluminescence and by the growth of cells in the presence of antibiotic. Toxicity parameter ЕС50 was 26.44 mg / l at 30 min exposure, and 0.30 mg / l during 8 hours of growth of bacteria. Dynamics of changes in toxicity, depending on the concentration of streptomycin in the long-term experiment, shows the difficulty in the choice of the end point of the experiment.