Antioxidant activities in vitro and hepatoprotective effects of Lysimachia clethroides Duby on CCl4-induced acute liver injury in mice

摘要

The antioxidant activity of the extracts of?Lysimachia clethroides?Duby (LC) was assayed by the methods of 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2-azino-bis(3-ethylbenzo-thiazoline-6-sulfonicacid) (ABTS)?in vitro. The ethyl acetate (LCEA) and?n-butanol extracts (LCBU) of?L. clethroides?were the higher antioxidant activity in DPPH and ABTS assay. LCEA had the highest antioxidant activity (DPPH: IC50?= 9.02 μg/ml, ABTS: IC50?= 7.43 μg/ml, respectively). Thus, hepatoprotective effect of the extracts of?L. clethroides?was evaluated on CCl4-induced acute liver injury mice. Intragastric administration of LCPE (500, 250 and 125 mg/kg body weight per day, respectively), LCEA (600, 300 and 150 mg/kg body weight per day, respectively) and LCBU (600, 300 and 150 mg/kg body weight per day, respectively) on CCL4-induced acute liver injury in mice for 8 days, the level of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in each treatment group significantly decreased (P 0.05), the other treatment groups had significant increase (P < 0.001, P < 0.01 and P < 0.05, respectively). The result showed that LC had a high antioxidant activities?in vitroand hepatoprotective effect on CCL4-induced acute liver injury in mice.