Isolation and molecular identification of antibiotic-producing actinomycetes from an old house in the medina of Fez, Morocco

摘要

Six?actinomycetes strains were?isolated?from soil?and?deteriorated?wood taken?from?an oldhouse?in?the?medina?of?Fez, Morocco. The antimicrobial activity?of these strains wastested?against?Gram-positive bacteria (Bacillus strains and Staphylococcus strains)?andGram-negative bacteria (Pseudomonas strain and Escherichia strain) and?Candida albicans.?Among the six isolates, five?showed?activity?against?at?least one?of the?testmicroorganisms. The activity was also found in organic extracts of the isolates. Furthermore, the organic extract from one strain was sensitive to temperature and proteinase K indicating that the active substance would be of protein nature. Molecularidentification of the isolates was performed on the basis of 16S rDNA sequence analysis. Three isolates were attributed to Streptomyces genus, namely:?Sj32 which could be identified as Streptomyces flavolimosus, Sj33 (Streptomyces spiroverticillatus)?and Sj38 (Streptomyces parvus)?whereas?Sj68 and Sj69 could be indicated as?Saccharothrixviolacea.