Quantitative Determination of Levofloxacin and Ambroxol Hydrochloride in Pharmaceutical Dosage Form by Reversed-Phase High Performance Liquid Chromatography

摘要

style="background-color:rgb(250, 250, 250); color:rgb(17, 17, 17); font-family:arial,verdana,helvetica,sans-serif; font-size:11.2px">The objective of this present work was to develop and validate analytical method for quantitative determination of Levofloxacin and Ambroxol hydrochloride in a new tablet formulation. Chromatographic separation of the two drugs were analyzed on a Hypersil BDS C18 column (25cm X 4.6mm, 5mm). The mobile phase constituted of Buffer: Acetonitirile: Methanol (650:250:100) with triethylamine and pH adjusted to 5.2 with dilute orthophosphoric acid was delivered at the flow rate 1.0 mL min-1 style="background-color:rgb(250, 250, 250); color:rgb(17, 17, 17); font-family:arial,verdana,helvetica,sans-serif; font-size:11.2px">. Detection was performed at 220 nm. Separation was completed within 10 min. Calibration curves were linear with coefficient correlation between 0.99 to 1.0 over a concentration range of 7 to 22 mg mL-1 style="background-color:rgb(250, 250, 250); color:rgb(17, 17, 17); font-family:arial,verdana,helvetica,sans-serif; font-size:11.2px"> of Levofloxacin and 50 to 150 mg/mL for Ambroxol hydrochloride respectively. The relative standard deviation (R.S.D) was found 2.0%.