Effects of Salinity on Growth, Feeding and the mRNA Expression of Na+/K+-ATPase and HSP 90 in Liza haematocheila

摘要

The effects of various salinities on growth, feeding and the mRNA expression of related genes in Liza haematocheilus were investigated. Fish were maintained at four salinities for 30 days to analyse the specific growth rate (SGR), the feeding rates (FR) of L. haematocheilus, and the mRNA expression of Na+/K+-ATPase (NKA) and Hsp 90 were measured at 0, 5, 10, 20 and 30 d, respectively. The results showed that the SGR of L. haematocheilus at 14 psu group was higher than the 2 and 42 psu group significantly; the FR of L. haematocheilus at 14 psu group was the highest and the 2 psu group was the lowest at 20 or 30 day; the NKA mRNA abundances in L. haematocheilus were increased significantly in the 2, 14 and 42 psu groups at 5 day (P < 0.05), and the lowest level emerged in the control group (28 psu), the same relationship was observed for Hsp 90 but the change scopes were smaller. The results indicate that the increased expression of NKA and Hsp 90 mRNA in L. haematocheilus is a part of the molecular responses to the osmotic stress of changes in salinity.