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Aromatase and Sex Steroid Receptors in Human Vena Cava

机译:人静脉中的芳香酶和性类固醇受体

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References(33) Cited-By(19) Among sex steroids, especially estrogen metabolism has been considered to play a role in the function and pathology of human veins. We investigated the expression and activity of the estrogen-producing enzyme aromatase and estrogen receptor (ER) in human vena cava to assess possible in situ biosynthesis of estrogens and their modes of action. We first examined aromatase expression by immunohistochemistry in human inferior vena cava obtained from 29 autopsy cases (11 males, 18 females, 63.6±3.0 years old). We then semiquantitated the level of aromatase mRNA by reverse transcriptase-polymerase chain reaction in 24 cases and aromatase activity by 3H-water assay in 15 cases to examine whether or not and in which cell types aromatase was expressed. We also studied alternative use of multiple exon is of its gene and immunolocalization of 17β-hydroxysteroid dehydrogenase type I (17β-HSD I), which converts estrone produced by aromatase to estradiol, a biologically active estrogen and ER. Aromatase and 17β-HSD I immunoreactivity were both detected in smooth muscle cells (SMC) of the media in all the cases and in endothelial cells (EC) in 20 and 22 cases, respectively. ER immunoreactivity was detected in SMC of vena cava in 21 cases. The amount of aromatase mRNA was significantly greater in the cases utilizing 1c (I.3) or id (P.II) of exon 1 (9 cases, 191.1±26.3 attomolg total RNA) than those utilizing 1b (I.4) as the promoter (14 cases, 50.6±13.0 attomolg total RNA) (p0.01). Significant correlation (p0.05) was observed between the amount of aromatase mRNA and aromatase activity in 15 cases examined. No significant correlation was detected between the amount of aromatase mRNA or aromatase labeling index and the ER status. These results suggest that estrone and estradiol are produced in the human vena cava and that their production is mediated by aromatase and 17β-HSD I, respectively but not all of these locally synthesized estrogens may not work directly in situ.
机译:参考文献(33)被引用的人(19)在性类固醇中,尤其是雌激素的代谢已被认为在人体静脉的功能和病理中起作用。我们调查了人类腔静脉中雌激素生成酶芳香酶和雌激素受体(ER)的表达和活性,以评估雌激素的可能原位生物合成及其作用方式。我们首先通过免疫组织化学检查了从29例尸检病例(11例男性,18例女性,63.6±3.0岁)获得的人下腔静脉中芳香化酶的表达。然后我们通过逆转录酶-聚合酶链反应对24例芳香化酶mRNA的水平进行了半定量,并通过3H-水分析对15例芳香化酶的活性进行了半定量,以检查芳香化酶是否表达以及在哪种细胞类型中表达。我们还研究了多种外显子的替代用途及其基因和I型17β-羟基类固醇脱氢酶(17β-HSDI)的免疫定位,该酶将芳香酶产生的雌酮转化为雌二醇,具有生物活性的雌激素和ER。在所有病例中,均在培养基的平滑肌细胞(SMC)中检测到芳香酶和17β-HSDI免疫反应,在20例和22例中,均检测到了内皮细胞(EC)。在21例腔静脉SMC中检测到ER免疫反应性。使用外显子1的1c(I.3)或id(P.II)的情况下(9例,总attomol / ng总RNA为191.1±26.3 at)的情况下,芳香化酶mRNA的量显着大于使用1b(I.4)的情况。作为启动子(14例,50.6±13.0 attomol / ng总RNA)(p <0.01)。在检查的15例患者中,芳香化酶mRNA的量与芳香化酶活性之间存在显着相关性(p <0.05)。在芳香化酶mRNA的量或芳香化酶标记指数与ER状态之间未发现显着相关性。这些结果表明雌激素和雌二醇在人腔静脉中产生,并且它们的产生分别由芳香酶和17β-HSDI介导,但是并非所有这些本地合成的雌激素都不能直接在原位起作用。

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