Persistence, period and precision of autonomous cellular oscillators from the zebrafish segmentation clock

摘要

The timing and pattern of gene activity in cells can be very important. For example, precise gene activity patterns in 24-hour circadian clocks help to set daily cycles of rest and activity in organisms. In such scenarios, cells often communicate with each other to coordinate the activity of their genes. To fully understand how the behavior of the population emerges, scientists must first understand the gene activity patterns in individual cells. Rhythmic gene activity is essential for the spinal column to form in fish and other vertebrate embryos. A group of cells that switch genes on/off in a coordinated pattern act like a clock to regulate the timing of the various steps in the process of backbone formation. However, it is not clear if each cell is able to maintain a rhythm of gene expression on their own, or whether they rely on messages from neighboring cells to achieve it. Now, Webb et al. use time-lapse videos of individual cells isolated from the tail of zebrafish embryos to show that each cell can maintain a pattern of rhythmic activity in a gene called Her1. In the experiments, individual cells were removed from zebrafish and placed under a microscope to record and track the activity of Her1 over time using fluorescent proteins. These experiments show that each cell is able to maintain a rhythmic pattern of Her1 expression on its own. Webb et al. then compared the Her1 activity patterns in individual cells with the Her1 patterns present in a larger piece of zebrafish tissue. The experiments showed that the rhythms in the individual cells are slower and less precise in their timing than in the tissue. This suggests that groups of cells must work together to create the synchronized rhythms of gene expression with the right precision and timing needed for the spinal column to be patterned correctly. In the future, further experiment with these cells will allow researchers to investigate the genetic basis of the rhythms in single cells, and find out how individual cells work together with their neighbors to allow tissues to work properly.