A comparison of callus induction in 4 Garcinia species

摘要

BackgroundThis research is intended to determine suitable types and concentrations of plant growth regulators (PGRs) to induce callus on stem and leaf sections of 4 species of the genusGarcinia, namely,Garcinia mangostana, Garcinia schomburgkiana,Garcinia cowa,andGarcinia celebica.The base medium was MS medium containing 30?g l-1sucrose, 0.5?g l-1polyvinylpyrrolidone (PVP), and 7?g l-1agar, and for the different treatments, PGRs were added to the medium as follows: thidiazuron (TDZ) at concentrations of 0, 0.1, 0.5, 1, and 2?mg l-1; 6-(3-hydroxybenzylamino) purine (meta-topolin) at concentrations of 0, 0.5, 2.5, and 5?mg l-1; 4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid (picloram) at concentrations of 0, 0.5, 2.5, and 5?mg l-1; and 2,4-dichlorophenoxyacetic acid (2,4-D) at concentrations of 0, 0.5, 1, 2, and 4?mg l-1. The occurrence of callus was observed after 4?weeks.ResultsA maximum of 100% and 93% ofG. mangostanaleaf explants formed callus in the 0.5?mg l-1and 1?mg l-1TDZ treatments, respectively, while 100% ofG. schomburgkianastem explants formed callus in the 1?mg l-1TDZ treatment and 89% ofG. schomburgkianaleaf explants formed callus in the 0.5?mg l-1picloram treatment. The highest callus induction rate forG. cowawas 62% in the 1?mg l-1TDZ treatment and forG. celebicawas 56% in the 0.5?mg l-1·mT-1treatment.ConclusionsFor all 4 species, the greatest amount of large nodular callus was observed in the TDZ treatments.White, friable callus was observed on most of the 2,4-D and picloram treatment groups. Mostmeta-topolin treatments resulted in minimal callus formation.How to cite:Suwanseree V, Phansiri S, Yapwattanaphun C. A comparison of callus induction in 4 Garcinia species. Electron J Biotechnol 2019;40.https://doi.org/10.1016/j.ejbt.2019.04.006.