Detection of rtN236T mutation associated with adefovir dipivoxil resistance in Hepatitis B infected patients with YMDD mutations in Tehran.

摘要

Background Objectives: The risk of adefovir dipivoxil resistance emergence has increased in lamivudine-resistant hepatitis B infected patients. The mutations known as causing adefovir resistance, rtN236T and rtA181V/T, are detected within the D and B functional domain of the HBV polymerase, respectively. In this study, we intended to determine the pre-existing adefovir-resistance mutations in patients infected with LAM resistant mutants prior to starting adefovir therapy.Material and Methods: The study included 30 patients with chronic hepatitis B with lamivudine resistance mutations in the YMDD motif that experienced viral breakthrough.Results: After alignment of protein coding sequences, the rtN236T mutation was observed in two (6.6 %) patients, while twenty-eight others had neither rtN236T, nor rtA181V/T mutation. All 30 patients were infected with genotype D of hepatitis B virus.Conclusions: The early detection of LAM-resistance mutations may allow a timely chance of therapy to avoid hepatitis flare- up. This data suggests that monitoring of ADV-resistance mutations in ADV na?ve patients can be considered in selecting the appropriate anti-viral regimen.