Characterization of the Full Length Coat Protein Gene of Iranian Grapevine fanleaf virus isolates, genetic variation and phylogenetic analysis

摘要

The full-length coat protein gene of Grapevine fanleaf virus (GFLV) isolates from Iran was characterized byreverse transcription polymerase chain reaction (RTPCR) and sequencing. The expected 1515 bp coatprotein (CP) gene amplicon was obtained for 16 isolates out of 89 that were identified by double antibodysandwich enzyme-linked immunesorbent assay (DASELISA) in a population of 330 symptomatic grapevineleaf samples. CP products of eight isolates were cloned and nucleotide sequences were determined.Parsimonious trees indicated that GFLV isolates from Iran formed a distinct cluster, suggesting an independent evolution.