Response of Lavandula vera to in vitro conditions

摘要

Tissue culture is fascinating, in that it allows for rapid propagation of genetically identical plants under controlled culture conditions. In the present study, response of Lavandula vera to in vitro conditions was observed for callus production and subsequent regeneration. Surface sterilization of seeds was carried out followed by inoculation on MS medium for callus production, supplemented with 0.2 mg L-1 BAP + 0.2 mg L-1 NAA. Induction of shooting from callus tissue was carried out using various plant growth regulators in different ratios - BAP (1 and 3 mg L-1 for 2% and 0.5, 1, 2 and 3 mg L-1 for 3% medium respectively), Indole-3-butyric acid or IBA (1, 2 and 3 mg L-1 1 for 2% medium), NAA (1 and 2 mg L-1 for 2% medium) and Kinetin (1 and 4 mg L-1 for 2% medium). Increased callus proliferation was observed as opposed to shoot induction, indicating a negative relationship between the two. From this, we can infer that alternative hormones in different concentrations may be required for regeneration of plantlets from callus tissue. Similar results were observed for increased levels of sucrose in combination with varying concentrations of BAP, which may be attributed to the type of plant growth regulator used, or different culture conditions. Production of blue pigments was observed with certain concentrations of BAP and with change in sucrose concentration, which may shed light on the requirements for secondary metabolite production, which would open up new avenues for obtaining compounds in vitro from callus tissue, thus preventing over-exploitation of these plants for their essential oils.