Brine Shrimp (Artemia salina) Bioassay of the medicinal plantPseudelephantopus spicatus from Iligan City, Philippines

摘要

Medicinal plants are commonly distributed in different regions of the Philippines. Based on ethnomedicinal significance, P.spicatus used in traditional medicine was collected and evaluated for biological activity using the Brine Shrimp Bioassay.Plant extracts were obtained through decoction, ethanolic extraction and extraction with ethanol-water. Four concentrations(10, 100, 500, 1000 ppm) of the P. spicatus extract were used. Mortality of the brine shrimp was observed after 6 hours and24 hours. The results showed that the decoction and ethanol-water extract were inactive against brine shrimp. However, theethanol extract showed a toxicity effect after 6h and 24h exposures with LC50 values at 944.07 and 266.07 ppm, respectively.Results indicate that the ethanol extract may have substances that are cytotoxic and that active components of the plant arebetter extracted with absolute ethanol than with hot water or mixture of ethanol and water. The active components presentmay have medicinal importance with no no adverse effects, and may support the therapeutic use of P. spicatus.