Statistical optimization of cellulase production in Pseudomonas spp and its application in bioethanol production

摘要

Cellulolytic microorganism converts cellulose into monomeric units which can be used for production of valuable products. This study demonstrates the production of cellulase by Pseudomonas spp VC14. Strain Pseudomonas spp VC14 cellulase activity was increased by optimization of physicochemical parameters by Plackett-Burman statistical design. Optimization showed NH4Cl and FeSO4 as significant components influencing cellulose production. On optimization the production of cellulase increased 5.41 fold (1.84 U/ml) within 24 hrs compared to unoptimized medium. Simultaneous saccharification and fermentation of reducing sugars with Saccharomyces cerevisae showed 5.1% bioethanol production. Thus, the results of the present work clearly revealed that cellulose from strain Pseudomonas spp VC14 can efficiently be used for bioethanol production.