Toxicological Studies of Herbal Anti-Tumor Extract (Uvaria Chamae) in Monosodium Glutamate and Tamoxifen Treated Sprague-Dawley Rat

摘要

Tumor cases are being linked to diet, lack of physical activity and overweight with Monosodium glutamate (MSG) and Tamoxifen (TAM) playing some roles. 45 female rats, weighing between 80g - 120g were divided into 3 main groups. 20 of the rats received MSG of 150mg/kg body weight, another 20 rats received TAM of 20mg/kg body weight and the remaining 5 rats were the control group. This was done for 30 days after which the rats were divided into 4 - subgroups and were treated for 21 days with ethanolic extract of Uvaria Chamae. They were then sacrificed, blood samples were collected into plain bottles and organs were harvested into universal bottle (fixed in 10% formal saline) for histopathology. It was observed that the amount of antibodies bound by the tumor markers was very significant in the MSG and TAM controls compared to the normal control (no tumor inducer rat). Administration of Uvaria Chamae extract was observed to cause very significant reduction in values of CA 15-3, CA-125 and CEA in both MSG and TAM groups compared to the untreated control. From the histopathology results, necrosis was observed in both treated and untreated groups of MSG and TAM but was not observed in the micrograph of the normal control. This study shows increase in the tumor markers values after induction with MSG and TAM, but Uvaria Chamae extract significantly reduced all values of the cancer tumor markers. The micrograph result showed damages done to the liver (by MSG and TAM), and this could not be corrected with Uvaria Chamaeextract.