Objective: The present study was conducted to evaluate the antimicrobial and antifongical activity of Hexane, ethyl acetate and methanol extracts of thirteen marine spices from Tunisian coastline (Chebba and Sfax): five pheophytea , five cholorophytea and three Magnoliophytea . Methods: These spices were tested against eight human pathogenic bacteria: Gram- ( Escherichia coli, DH5 (alpha), listeria monocytogéne, Salmonella enterica, Agrobacterium tumefaciens , Pseudomonas aerigunosa) , Gram+ ( Staphylococus aureus, Micrococcus luteus) , and two human pathogenic yeast ( Candida tropicalis , saccharomyces cerevisiae ) and a fungi ( Aspergillus niger) , using the agar disk diffusion assay method. Results: The results of thirteen marine spices extracts collected from the coast of Tunisia have shown significant antimicrobial activity and the maximum inhibitory activities were observed in the brown algae, it was more active on bacteria than the fungi. Conclusion: The results of the present study revealed that the seaweeds and magnoliophytea from Tunisia appear to have immense potential as a source of antibacterial and antifongical compounds, they could be used in treating diseases caused by these organisms test. Keywords: Antibacterial activity, Antifungal activity, Disc diffusion method.
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