COMPARATIVE PHYTOCHEMICAL ANALYSIS OF CUSCUTA REFLEXA PARASITE GROWN ON CASSIA FISTULA AND FICUS BENGHLENSIS BY GC-MS

摘要

Objective: The present study was aimed to determine the phytochemicals present in Cuscuta reflexa parasite grown on two different hosts. The comparative GC-MS analysis of ethyl acetate extract of Cuscuta reflexa grown on Cassia fistula and Ficus benghalensis was performed. Method: Powdered sample of Cuscuta reflexa from both the host trees was extracted with petroleum ether, ethyl acetate, methanol and water. 50μl of ethyl acetate extract was dissolved in 2ml of methanol and kept in ultrasonic bath for 15mins and centrifuged for 10mins at 6000 rpm and supernatant was analyzed by GC-MS Result: This analysis revealed that 1, 2, 3-propanetriol 1-acetate, benzofuran 2, 3-dihydro, glycerol, 1, 2-diacetate, 2-methoxy-4-vinylphenol and triacetin are present in Cuscuta reflexa grown on both the host trees. Cuscuta reflexa grown on Cassia fistula showed the presence of 1H-1,2,4-triazol-5-amine-1-ethyl, D-glucitol-4-O-hexyl, 3,4,5-trimethoxy cinnamic acid, 3,6-dimethoxy phenanthrene and 3,5-di tert butyl 4-hydroxyanisol while these compounds are absent in Cuscuta reflexa grown on Ficus benghalensis. Vanillin, 3-aminopyrrolidine, cetene, sarcosine N-isobutyryl-tetra decyl ester, 4-((1E)-3-hydroxy-1-propenyl)-2-methoxyphenol, 1,5-diphenyl-2H-1,2,4 triazoline-3-thione, 1-octadecene, heptanamide,N-(1-cyclohexylethyl)-2-methyl, scoparone, 3'methyl-2-benzyledine-coumaran-3-one are present only in Cuscuta reflexa grown on Ficus benghalensis while absent in Cuscuta reflexa grown on Cassia fistula. Conclusion: This comparative study confirms that phytochemicals present in Cuscuta reflexa parasite depends on nature of host