PRODUCTION OF ANTIMICROBIAL AND ANTICANCER FOM FEATHER-KERATINOLYTIC NOCARDIOPSIS SP. 28ROR AS A NOVEL STRAIN USING FEATHER MEAL MEDIUM

摘要

Objective: Production of bioactive secondary metabolites from a feather-degrading actinobacterial species using feather meal medium. Methods: Protease producer actinobacterial isolates (22) recovered from farm soil, poultry farm soil and feather wastes were used to test the antagonistic effect on pathogenic bacteria and fungi, including Staphylococcus aureus, Escherichia coli , Microsporum canis and Trichophyton mentagrophyte using Mueller-Hinton agar and potato dextrose agar (PDA) media. The best isolate was used to produce the active metabolites in feather meal medium composed from (g/l) 10 feather meal, 5 sucrose and 0.3 l cement extract were dissolved in tap water, in addition to the standard medium composting of (g/l) glucose (1%), tryptone (1%), KH 2 PO 4 (0.07%) and K 2 HPO 4 (0.14%) were dissolved in distilled water, both of them at initial pH 9. The secondary metabolites were partially purified by Sephadex LH20 column and the antimicrobial activity and cytotoxic activity were assayed. Results: 31.82% of isolates inhibited the growth of both bacterial and fungal test organisms and the best one was Nocardiopsis sp . 28ROR (GenBank: KC702802.1) at a significant level P ? 0.05. It produced bioactive metabolites in both feather meal broth and the standard media. The partially purified metabolites inhibit the breast cancer cell line MCF-7 (51%) and normal hepatic cell line WRL-68 (9%), in addition to inhibiting S. aureus and Trichophyton menta agrophyte growth. Conclusion: The actinobacteria has vast abilities to degrade very complex wastes and converted to simple constituents to reprocess in other industries. So the Nocardiopsis sp. 28ROR was a novel strain produced anticancer, antimicrobial substances using feather meal medium as a cheap waste medium.