COMPARATIVE STUDY OF AQUEOUS EXTRACT OF MOMORDICA CHARANTIA SEEDS WITH SYNTHETIC INSULIN SENSITIZERS ON BLOOD GLUCOSE LEVELS AND BODY WEIGHT IN ALBINO RATS

摘要

Objective: To evaluate the effect of aqueous extract of Momordica charantia seed in combination with an insulin sensitizer metformin on blood glucose levels and body weight compared to metformin plus pioglitazone. Methods: Rats were divided into 5 groups of 6 animals each. Metformin and Pioglitazone were given at doses of 1250mg/kg b. w/day and 75mg/kg b. w/day. Momordica Charantia Seed Extract (MCSE) was administered at a dose of 2500mg/kg b. w/day. All these drugs were administered orally once daily for a period of 7 days (total study duration). Injection Dexamethasone (8mg/kg) was given intraperitoneally to all the 3 test groups during the last 3 days of study to induce a state of insulin resistance. Blood glucose levels and body weight were measured before starting the study, before starting Dexamethasone (Day 5), and at the end of the study (Day 7). Data were analyzed using one-way analysis of variance (ANOVA) followed by Dunnet test. Results: In case of Dexamethasone induced diabetic model, the combination of Metformin and MCSE exhibited significant reduction in blood glucose ( P = 0.019) and body weight ( P = 0.004) as compared to Metformin and Pioglitazone. Conclusion: The aqueous extract of Momordica charantia seed has potent blood glucose lowering activity in dexamethasone induced diabetes in rat.