HPTLC FINGERPRINT PROFILE AND EVALUATION OF ANTIOXIDANT POTENTIAL OF FLAVONOID RICH FRACTION FROM NYCTANTHES ARBOR-TRISTIS L. LEAVES

摘要

Objective: The present study was carried out to obtain the flavonoid rich extract from the leaves of Nyctanthes arbor-tristis Linn. for evaluation of its antioxidant activity. Materials and Methods: In the present study, Supercritical Fluid (SCF) extraction of plant material was carried out at optimized conditions of temperature 40°C, pressure 24.51 MPa, CO2flow rate 2ml/min and co-solvent (ethanol) of 8.25%. Total phenolic Content (TPC) and Total Flavonoid Content (TFC) of the SCF extract were determined by Folin-Ciocalteau and Aluminium Chloride colorimetric methods, respectively. HPTLC fingerprint analysis was also done for different fractions obtained from the crude extract. Antioxidant activity of the SCF crude and ethyl acetate fractions was evaluated by DPPH and Reducing Power Assay. Results: Extraction yield of SCF extract under optimized conditions was found to be 69.85 + 1.25. TPC and TFC were found to be 304.5 +1.70 mg GAE/g and 174.85 + 1.36 mg QE/g respectively. The optimized solvent system developed for TLC was Ethyl acetate: Methanol: Formic acid: Water [50:2:3:6]. The IC50value of SCF crude and ethyl acetate fractions was found to be 62.166 + 1.86 and 55.387 + 1.26μg/mL, respectively. Conclusion: The results of this study show that the leaf extract of N. arbor-tristis can be used as natural source of antioxidants