ABSORPTION CORRECTION METHOD FOR THE SIMULTANEOUS ESTIMATION OF AMLODIPINE BESYLATE, VALSARTAN AND HYDROCHLOROTHIAZIDE IN BULK AND IN COMBINED TABLET DOSAGE FORM

摘要

.new,.simple,.accurate.and.sensitive.UV.‐.spectrophotometric.absorption.correction.method.has.been.developed.for.simultaneous.determination.of.amlodipine. besylate,. valsartan. and.hydrochlorothiazide. in. bulk. and. in.combined. tablet.dosage. form..Methanol. and.distilled. water. were.used. as.solvents..The.wavelengths.selected.for.the.analysis.were.365.nm,.250.nm.and.315.nm.for.amlodipine.besylate,.valsartan.and.hydrochlorothiazide,.respectively..Beer's.law.obeyed.the.concentration.range.of.1.–.32.μg/.ml,.4.–.40.μg/.ml.and.2.–.20.μg/.ml.for.amlodipine.besylate,.valsartan.and.hydrochlorothiazide,.respectively..The.percentage.recovery.was.found.in.the.range.of.100.42.‐.101.27%.for.amlodipine.besylate,.100.51.‐.101.40%.for.valsartan.and.99.25.‐.100.35%.for.hydrochlorothiazide..The. developed.method.was.validated.statistically.and.by.recovery.studies..The.%.RSD.value.was.found.to.be.less.than.2..Thus.the.proposed.method.was.simple,.precise,.economic,.rapid.and.accurate.and.can.be.successfully.applied.for.simultaneous.determination.of.amlodipine.besylate,.valsartan.and.hydrochlorothiazide.in.bulk.and.in.combined.tablet.dosage.form