EFFECT OF ETHANOL STEM BARK EXTRACT OF BERLINIA GRANDIFLORA HUTCH AND DALZ ON MARKER LIVER ENZYMES IN RATS TREATED WITH CCl4

摘要

Objective: The objective of this work was to validate the traditional claim of the use of the stem bark of Berlinia grandiflora in treating liver problems by investigating the effect of its ethanol stem bark extract on maker liver enzymes in vivo . Methods: Groups of Wistar albino rats (6 in each group) were daily treated with CCl 4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously), followed by oral administration of ethanol stem bark extract of Berlinia grandiflora (ESBG) at doses 100, 300 and 900 mg/kg b.w. respectively, 3 h after the administration of the CCl 4 . Serum biochemical parameters were measured 24 h at the end of the 7-day treatment period and compared to a group intoxicated with CCl 4 (2 ml/kg b.w., diluted with olive oil 1:1 v/v, intravenously) alone. Results: Treatment of rats with ESBG (at doses 100, 300 and 900 mg/kg b.w. orally) reduced the impact of CCl 4 -induced hepatotoxicity on liver maker enzymes Alanine aminotransferase (ALT), Aspartate aminotransferase (AST), Alkaline phosphatase (ALP) and total bilirubin (TB), with the 900 mg/kg b.w. showing a significant reduction (p0.05) compared to the group intoxicated with CCl 4 . The extract reversed the impact of the CCl 4 -induced hepatotoxicity by increasing the concentration of the serum Total protein (TP) and albumin (ALB), with the 900 mg/kg b.w. dose showing a significant increment (p0.05) compared to the group intoxicated with the CCl 4 . Conclusion: The investigation of the effect of Berlinia grandiflora on CCl 4 induced liver damage revealed that the ethanol extract of the stem bark of the plant was able to reverse the hepatotoxicity, with the 900 mg/kg b.w. dose showing a significant activity.