A newly isolated green alga, Pediastrum duplex Meyen, from Thailand with efficient hydrogen production

摘要

Biofuels are gaining attention worldwide as a way to reduce the dependence on fossil fuels. Biological Hydrogen (H_2) production is considered the most environmentally friendly route of producing H_2, fulfilling the goals of recycling renewable resources and producing clean energy. It has attracted global attention because of its potential to become an inexhaustible, low cost, renewable source of clean energy and appears as an alternative fuel. H_2 production processes offer a technique through which renewable energy sources like biomass can be utilized for the generation of the cleanest energy carrier for the use of mankind. This paper presents laboratory results of biological production of hydrogen by green alga was isolated from fresh water fish pond in Sansai, Chiang Mai province, Thailand. Under light microscope, this green alga was identified as belonging to the genus Pediastrum and species P. duplex Meyen. The successful culture was established and grown in poultry litter effluent medium (PLEM) under a light intensity of 37.5 μmol~(-1)m~2 sec~(-1) and a temperature of 25℃. The nutrient requirements and process conditions that encourage the growth of dense and healthy algal cultures were explored. The highest H_2 was produced when cultivated cells in PLEM for 21 hours under light and then incubated under anaerobic adaptation for 4 hours.