Long-lasting bactericidal activity through selective physical puncture and controlled ions release of polydopamine and silver nanoparticles–loaded TiO 2 nanorods in vitro and in vivo

摘要

Background: Titanium (Ti) implant-associated infection, which is mostly caused by bacterial adhesion and biofilm formation, may result in implant failure and secondary surgery. Thus it is an urgent issue to prevent bacterial infections at the earliest step. Purpose: To develop a novel surface strategy of polydopamine (PDA) and silver (Ag) nanoparticle-loaded TiOsub2/sub nanorods (NRDs) coatings on Ti alloy. Materials and methods: Ag-TiOsub2/sub@PDA NRDs was fabricated on Ti alloy by hydrothermal synthesis. The antibacterial activity of Ag-TiOsub2/sub@PDA NRDs against Escherichia coli and methicillin-resistant Staphylococcus aureus were tested by FE-SEM, Live/Dead staining, zone of inhibition, bacteria counting method and protein leakage analysis in vitro . In addition, an implant infection model was conducted and the samples were tested by X-ray, Micro-CT and histological analysis in vivo . Besides, cell morphology and cytotoxicity of Mouse calvarial cells (MC3T3-E1) were characterized by FE-SEM, immunofluorescence and CCK-8 test in vitro.