Evaluation of Anticancer Properties against Ehrlich Ascites Carcinoma (EAC) Cell Line, Cytotoxic and Analgesic Activity of Methanol Extract of Hibiscus moscheutos in Swiss Albino Mice

摘要

The present study has been designed to explore the analgesic, cytotoxic & in vivo anticancer activity of methanol extract of Hibiscus moscheutos against Ehrlich Ascites Carcinoma (EAC) in Swiss albino mice. Most of the natural sources of anticancer drugs are designed to evaluate the selective toxicity to rapidly dividing cells. The in vivo anticancer activity has been evaluated against EAC cells in Swiss albino mice by monitoring parameters including tumor cell growth inhibition, apoptosis assessment, tumor weight measurement, mean survival time and hematological parameters. This study indicated that dose of 400 mg/kg/day (i.p) that significantly increase life span, tumor cell breakdown, & decrease tumor weight, tumor cell growth rate compared to control group. Vincristine sulfate was used as a positive control (0.3 mg /kg). At the dose of 400 mg/kg/day(i.p) hematological parameters such as hemoglobin content, RBC, WBC, Lymphocytes, Neutrophils, Monocytes, Cholesterol, Triglyceride & Blood urea were significantly restored. In analgesic bioassay, the efficacy of Hibiscus moscheutos was 65.44% after orally administration (400 mg/kg). This outcome makes Hibiscus moscheutos as a possible potential candidate for using as anticancer & analgesic agent. In cytotoxic test, it showed significant result at 1479.10 μg/ml.